Diamide resistance: 10 years of lessons from Lepidopteran pests

Diamide insecticides selectively acting on insect ryanodine receptors (RyR) were launched to the market more than 10 years ago, particularly targeted for the control of lepidopteran pest species in diverse agronomic and horticultural cropping systems. They are now globally registered in many countries and provide reliable control levels in most settings. However, their frequent application, due to alternative mode of action chemistries often not providing sufficient levels of control, has resulted in the selection of diamide resistance in some of the world’s most destructive lepidopteran species, including populations of diamondback moth, tomato leafminer, rice stem borer and more recently beet armyworm. High levels of diamide resistance, compromising diamide efficacy at recommended field rates, has been shown to be conferred by RyR target-site mutations affecting diamide binding. The present work reviews the global status of diamide insecticide resistance in lepidopteran pests, with special reference to RyR target-site alterations. Furthermore, we discuss principles enabling the prediction of the impact and spread of diamide resistance, based on population genetics and associated fitness costs as influenced by the known target-site mutations recently described. In this context, we reiterate calls by the Insecticide Resistance Action Committee to implement effective diamide insecticide resistance management by following a three-step strategy of resistance identification, tracking and prediction according to the protocols discussed in this article

Heavy Ion Physics at RHIC

The status of the physics of heavy ion collisions is reviewed based on measurements over the past 6 years from the Relativistic Heavy Ion Collider (RHIC) at Brookhaven National Laboratory. The dense nuclear matter produced in Au+Au collisions with nucleon-nucleon c.m. energy $\sqrt{s_{NN}}=200$ GeV at RHIC corresponds roughly to the density and temperature of the universe a few microseconds after the `big-bang' and has been described as "a perfect liquid" of quarks and gluons, rather than the gas of free quarks and gluons, ``the quark-gluon plasma" as originally envisaged. The measurements and arguments leading to this description will be presented.Comment: 31 pages, 23 figures, Proceedings of Symposium "50+ Years of High Energy Physics at UB", University at Buffalo, NY, October 20-21,200

Proton stopping in C+C, d+C, C+Ta and d+Ta collisions at 4.2A GeV/c

The shape of proton rapidity distributions is analysed in terms of their Gaussian components, and the average rapidity loss is determined in order to estimate the amount of stopping in C+C, d+C, C+Ta and d+Ta collisions at 4.2A GeV/c. Three Gaussians correspond to the nuclear transparency and describe well all peripheral and also C+C central collisions. Two-component shape is obtained in case of d+C and C+Ta central collisions. Finally one Gaussian, found in d+Ta central collisions, corresponds to the full stopping. The calculated values of the average rapidity loss support the qualitative relationship between the number of Gaussian components and the corresponding stopping power. It is also observed, in central collisions, that the average rapidity loss increases with the ratio of the number of target and the number of projectile participants.Comment: 9 pages REVTeX, 1 PS figure replaced, to be published in Phys.Rev.

Break-up stage restoration in multifragmentation reactions

In the case of Xe+Sn at 32 MeV/nucleon multifragmentation reaction break-up fragments are built-up from the experimentally detected ones using evaluations of light particle evaporation multiplicities which thus settle fragment internal excitation. Freeze-out characteristics are extracted from experimental kinetic energy spectra under the assumption of full decoupling between fragment formation and energy dissipated in different degrees of freedom. Thermal kinetic energy is determined uniquely while for freeze-out volume - collective energy a multiple solution is obtained. Coherence between the solutions of the break-up restoration algorithm and the predictions of a multifragmentation model with identical definition of primary fragments is regarded as a way to select the true value. The broad kinetic energy spectrum of $^3$He is consistent with break-up genesis of this isotope.Comment: 17 pages, 5 figure

Neofunctionalization of Duplicated P450 Genes Drives the Evolution of Insecticide Resistance in the Brown Planthopper

This is the final version of the article. Available from Elsevier via the DOI in this record.Gene duplication is a major source of genetic variation that has been shown to underpin the evolution of a wide range of adaptive traits [1, 2]. For example, duplication or amplification of genes encoding detoxification enzymes has been shown to play an important role in the evolution of insecticide resistance [3–5]. In this context, gene duplication performs an adaptive function as a result of its effects on gene dosage and not as a source of functional novelty [3, 6–8]. Here, we show that duplication and neofunctionalization of a cytochrome P450, CYP6ER1, led to the evolution of insecticide resistance in the brown planthopper. Considerable genetic variation was observed in the coding sequence of CYP6ER1 in populations of brown planthopper collected from across Asia, but just two sequence variants are highly overexpressed in resistant strains and metabolize imidacloprid. Both variants are characterized by profound amino-acid alterations in substrate recognition sites, and the introduction of these mutations into a susceptible P450 sequence is sufficient to confer resistance. CYP6ER1 is duplicated in resistant strains with individuals carrying paralogs with and without the gain-of-function mutations. Despite numerical parity in the genome, the susceptible and mutant copies exhibit marked asymmetry in their expression with the resistant paralogs overexpressed. In the primary resistance-conferring CYP6ER1 variant, this results from an extended region of novel sequence upstream of the gene that provides enhanced expression. Our findings illustrate the versatility of gene duplication in providing opportunities for functional and regulatory innovation during the evolution of an adaptive trait.This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement n°646625), the Biotechnology and Biological Sciences Research Council of the UK (BB/G023352/1), and Bayer Crop Science

Combining Parton Showers with Next-to-Leading Order QCD Matrix Elements in Deep-Inelastic eP Scattering

We have implemented a systematic procedure for combining parton shower algorithms with next-to-leading order QCD calculations for the case of jet production in deep-inelastic electron-proton scattering. Using this method we have computed inclusive jet cross sections and jet shapes for the case of single-jet production and compared them to data from the ZEUS collaboration at HERA. We found good agreement between the data and our calculations, both for the jet shapes and the inclusive spectra.Comment: 13 pages, 4 eps figure

Chimeric Investigations into the Diamide Binding Site on the Lepidopteran Ryanodine Receptor

Alterations to amino acid residues G4946 and I4790, associated with resistance to diamide insecticides, suggests a location of diamide interaction within the pVSD voltage sensor-like domain of the insect ryanodine receptor (RyR). To further delineate the interaction site(s), targeted alterations were made within the same pVSD region on the diamondback moth (Plutella xylostella) RyR channel. The editing of five amino acid positions to match those found in the diamide insensitive skeletal RyR1 of humans (hRyR1) in order to generate a human−Plutella chimeric construct showed that these alterations strongly reduce diamide efficacy when introduced in combination but cause only minor reductions when introduced individually. It is concluded that the sites of diamide interaction on insect RyRs lie proximal to the voltage sensor-like domain of the RyR and that the main site of interaction is at residues K4700, Y4701, I4790 and S4919 in the S1 to S4 transmembrane domains

Elliptical flow -- a signature for early pressure in ultrarelativistic nucleus-nucleus collisions

Elliptical energy flow patterns in non-central Au(11.7AGeV) on Au reactions have been studied employing the RQMD model. The strength of these azimuthal asymmetries is calculated comparing the results in two different modes of RQMD (mean field and cascade). It is found that the elliptical flow which is readily observable with current experimental detectors may help to distinguish different reasonable expansion scenarios for baryon-dense matter. The final asymmetries are very sensitive to the pressure at maximum compression, because they involve a partial cancelation between early squeeze-out and subsequent flow in the reaction plane. This cancelation can be expected to occur in a broad energy region covered by the current heavy ion fixed-target programs at BNL and at CERN.Comment: 14 pages LaTeX including 3 postscript figure

Effects of Compression and Collective Expansion on Particle Emission from Central Heavy-Ion Reactions

Conditions under which compression occurs and collective expansion develops in energetic reactions of heavy nuclei, are analyzed, together with their effects on emitted light baryons and pions. Within transport simulations, it is shown that shock fronts perpendicular to beam axis form in head-on reactions. The fronts separate hot compressed matter from normal. As impact parameter increases, the angle of inclination of the fronts relative to beam axis decreases, and in-between the fronts a weak tangential discontinuity develops. Hot matter exposed to the vacuum in directions perpendicular to shock motion (and parallel to fronts), starts to expand sideways, early within reactions. Expansion in the direction of shock motion follows after the shocks propagate through nuclei, but due to the delay does not acquire same strength. Expansion affects angular distributions, mean-energy components, shapes of spectra and mean energies of different particles emitted into any one direction, and further particle yields. Both the expansion and a collective motion associated with the weak discontinuity, affect the magnitude of sideward flow within reaction plane. Differences in mean particle energy components in and out of the reaction plane in semicentral collisions, depend sensitively on the relative magnitude of shock speed in normal matter and speed of sound in hot matter.Comment: 71 pages, 33 figures (available on request), report MSUCL-94

Event Anisotropy in High Energy Nucleus-Nucleus Collisions

The predictions of event anisotropy parameters from transport model RQMD are compared with the recent experimental measurements for 158$A$ GeV Pb+Pb collisions. Using the same model, we study the time evolution of event anisotropy at 2$A$ GeV and 158$A$ GeV for several colliding systems. For the first time, both momentum and configuration space information are studied using the Fourier analysis of the azimuthal angular distribution. We find that, in the model, the initial geometry of the collision plays a dominant role in determining the anisotropy parameters.Comment: 18 pages, 7 figures, 2 table